Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis

Table 2 Sequence and binding scores of peptides to HLA-B27 and HLA-A2

Name of peptide	Sequence of peptide	Binding score^a	Binding score^b	Refolding	Reference
HLA-B27/EBNA (258–266) (EBV)	RRIYDLIEL	28	2000	++	[24]
HLA-A2/BMLF1 lytic antigen peptide 280–288 (EBV)	GLCTLVAML	29	6000	++	[21]
HLA-B27/Influenza NP 383–391	SRYWAIRTR	26	1500	+	[32]
HLA-B27/Chlamydia peptide 8	NRFSVAYML	26	10,000	++	[10]
HLA-B27/Chlamydia peptide 68	NRAKQVIKL	26	2000	(+)	[10]
HLA-B27/Chlamydia peptide 80	IRMFKILPL	26	2000	+	[10]
HLA-B27/Chlamydia peptide 131	KRLAETLAL	26	6000	(+)	[10]
HLA-B27/Chlamydia peptide 133	IRSSVQNKL	27	2000	(+)	[10]
HLA-B27/Chlamydia peptide 138	ARKLLLDNL	26	2000	++	[10]
HLA-B27/Chlamydia peptide 144	MRDHTITLL	25	2000	-	[10]
HLA-B27/Chlamydia peptide 145	DRLALLANL	27	200	+	[10]
HLA-B27/Chlamydia peptide 146	YRLLLTRVL	25	600	(+)	[10]
HLA-B27/Chlamydia peptide 194	EREQTLNQL	25	200	-	[10]
HLA-B27/Chlamydia peptide 195	NRELIQQEL	25	2000	(+)	[10]
HLA-B27/Chlamydia peptide 196	ERFLAQEQL	27	1000	(+)	[10]

Refolding rates are designated as follows: ++, more than 75% soluble HLA-B27 monomer in gel-filtration analysis after refolding and biotinylation; +, more than 50% refolding; (+), more than 10% refolding; -, less than 10% refolding.
^aSYFPEITHI, HG Rammensee, University of Tübingen [23].
^bHLA-peptide binding motifs (the BioInformatics and Molecular Analysis Section; http://bimas.dcrt.nih.gov/molbio/hla_bind/). EBV, Epstein–Barr virus.

ISSN: 1478-6362