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Table 2 Sequence and binding scores of peptides to HLA-B27 and HLA-A2

From: Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis

Name of peptide Sequence of peptide Binding scorea Binding scoreb Refolding Reference
HLA-B27/EBNA (258–266) (EBV) RRIYDLIEL 28 2000 ++ [24]
HLA-A2/BMLF1 lytic antigen peptide 280–288 (EBV) GLCTLVAML 29 6000 ++ [21]
HLA-B27/Influenza NP 383–391 SRYWAIRTR 26 1500 + [32]
HLA-B27/Chlamydia peptide 8 NRFSVAYML 26 10,000 ++ [10]
HLA-B27/Chlamydia peptide 68 NRAKQVIKL 26 2000 (+) [10]
HLA-B27/Chlamydia peptide 80 IRMFKILPL 26 2000 + [10]
HLA-B27/Chlamydia peptide 131 KRLAETLAL 26 6000 (+) [10]
HLA-B27/Chlamydia peptide 133 IRSSVQNKL 27 2000 (+) [10]
HLA-B27/Chlamydia peptide 138 ARKLLLDNL 26 2000 ++ [10]
HLA-B27/Chlamydia peptide 144 MRDHTITLL 25 2000 - [10]
HLA-B27/Chlamydia peptide 145 DRLALLANL 27 200 + [10]
HLA-B27/Chlamydia peptide 146 YRLLLTRVL 25 600 (+) [10]
HLA-B27/Chlamydia peptide 194 EREQTLNQL 25 200 - [10]
HLA-B27/Chlamydia peptide 195 NRELIQQEL 25 2000 (+) [10]
HLA-B27/Chlamydia peptide 196 ERFLAQEQL 27 1000 (+) [10]
  1. Refolding rates are designated as follows: ++, more than 75% soluble HLA-B27 monomer in gel-filtration analysis after refolding and biotinylation; +, more than 50% refolding; (+), more than 10% refolding; -, less than 10% refolding.
  2. aSYFPEITHI, HG Rammensee, University of Tübingen [23].
  3. bHLA-peptide binding motifs (the BioInformatics and Molecular Analysis Section; http://bimas.dcrt.nih.gov/molbio/hla_bind/). EBV, Epstein–Barr virus.
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