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Table 2 Sequences of the gene-specific oligonucleotide primers and probes for real-time reverse transcriptase polymerase chain reaction

From: Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction

Primer and probe

Sequence

Position

HAS-1

855F

GACTCCTGGGTCAGCTTCCTAAG

855 – 877

 

995R

AAACTGCTGCAAGAGGTTATTCCT

995 – 972

 

probe

TATCCTGCATCAGCGGTCCTCTAGGC

940 – 965

HAS-2

20F

CTATGCTTGACCCAGCCTCATC

20 – 41

 

149R

ACACTGCTGAGGAATGAGATCCA

149 – 127

 

MGB probea

AGATGTCCAGATTTTA

96 – 111

HAS-3

888F

TGTCCAGATCCTCAACAAGTACGA

888 – 911

 

1005R

AATACACTGCACACAGCCAAAGTAG

1005 – 981

 

probe

TCATGGATTTCCTTCCTGAGCAGCGT

913 – 938

HYAL-1

1561F

AGTGGTGCTCTGGGTGAGCT

1561 – 1580

 

1667R

TGGTCACGTTCAGGATGAAGG

1667 – 1647

 

probe

CCAAGGAATCATGTCAGGCCATCAAGG

1596 – 1622

HYAL-2

1069F

CGCAGCTGGTGTCATCCTCT

1069 – 1088

 

1159R

CAGCAGCCGTGTCAGGTAATC

1159 – 1139

 

probe

TACACCACAAGCACGGAGACCTGCC

1103 – 1127

HYAL-3

1130F

GCCTCACACACCGGAGATCT

1130 – 1149

 

1202R

GCTGCACTCACACCAATGGA

1202 – 1183

 

probe

TCCTGTCCCAGGATGACCTTGTGCA

1157 – 1181

  1. aMinor groove binder (MGB) enhances the melting temperature of the probe (see Materials and methods). HAS, hyaluronan synthase; HYAL, hyaluronidase.