Figure 5

Activity of procoagulant and TF and concentrations of plasma TAT in mice with induced arthritis (CIA or AIA) and controls. (a) PCA (expressed in seconds taken for thrombus formation) in tissue extracts. Arthritic tissue extracts (A) were prepared from arthritic, swollen paws at day 42 after the first immunization (CIA model) or from arthritic right knee joints at day 3 after arthritis induction (AIA model). Nonarthritic (NA) tissue extracts were from paws of normal, nonimmunized mice (CIA model) or normal left knees (contralateral to induced arthritis; AIA model). (b) TF activity in arthritic (A) and nonarthritic (NA) tissue extracts (the same extracts as were used for PCA), measured using a chromogenic assay. (c) Plasma concentrations of TAT complexes as determined in arthritic (A) mice in comparison with nonimmunized, nonarthritic (NA) controls. Citrated plasmas from arthritic mice were prepared at day 42 after the first immunization (CIA model) or at day 3 after arthritis induction (AIA model). Values are means ± SEM of at least four samples from four mice. –^*P < 0.05, arthritic versus nonarthritic values.