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Table 1 Deficient induction of IL-4 producers from synovial precursors

From: Rheumatoid synovial CD4+ T cells exhibit a reduced capacity to differentiate into IL-4-producing T-helper-2 effector cells

  Percentage of cytokine-producing cells
  RAPB RASF
Addition IL-2 IL-2 + IFN-γ IFN-γ IL-4 IL-2 IL-2 + IFN-γ IFN-γ IL-4
Nil 19 24 34 2 4 27 60 0
Anti-IFN-γ 23 25 28 1 7 33 47 0
IL-4 32 23 25 14 5 29 49 0
Anti-IFN-γ + IL-4 41 16 20 23 4 23 54 0
  1. CD45RO+, CD4+ memory effector T cells were obtained from RA matching peripheral blood and synovial fluid. T cells were cultured for 1 week with OKT3 (0.01 μg/ml) and anti-CD28, as described in Materials and methods. Where indicated, the cultures contained saturating concentrations of anti-IFN-γ antibody and/or IL-4. Afterward, the cells were washed, rested for 2.5 days, and restimulated for 6 h with mitogen to assess intracellular cytokines by flow cytometry; 104 cells were analyzed for each sample. The percentage of cytokine-expressing cells for each sample is shown. Similar results were obtained in at least two independent experiments. No significant difference (P > 0.05) was observed in the percentage of synovial IL-4-producing effector cells in the absence or presence of anti-IFN-γ antibody and/or IL-4.