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Table 1 Composition of rheumatoid arthritis (RA)-synovial fibroblasts (SFB), osteoarthritis (OA)-SFB, and normal skin-FB, as determined by flow cytometry for various numbers of patients/donors

From: Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture — primary culture cells markedly differ from fourth-passage cells

  Percent of positive cells Mean fluorescence intensity
Composition Mean ± SEM Range Mean ± SEM Range
(a) Primary-culture RA synovial cells
   Thy-1 (CD90) (n = 4) 62.0 ± 14.6 24–93 152 ± 49 46–283
   CD14 (n = 4) 14.8 ± 6.0 2–25 230 ± 87 64–445
(b)RA-SFB (negative isolation from primary culture; negative fraction)
   Thy-1 (n = 9) 73.8 ± 9.6 21–99 109 ± 20 53–222
   Prolyl-4-hydroxylase (n = 9) 84.8 ± 2.9 66–95 164 ± 45 20–391
   CD14 (n = 9) 0.7 ± 0.2 0.1–1.7 62 ± 12 30–126
   CD68 (mAb PG-M1) (n = 5) 1.7 ± 0.6 0.4–3.2 120 ± 78 10–430
(c)RA-SFB (fourth-passage; isolated cells)
   Thy-1 (n = 3) 86.6 ± 4.6 79–95 41 ± 7 27–53
   Prolyl-4-hydroxylase (n = 3) 98.7 ± 0.8 97–100 164 ± 62 82–285
   CD14 (n = 3) 0.6 ± 0.2 0.2–1.0 50 ± 30 20–110
(d)RA-SFB (fourth-passage; conventionally passaged cells)
   Thy-1 (n = 6) 85.9 ± 3.9 70–95 95 ± 15 43–167
   Prolyl-4-hydroxylase (n = 6) 98.3 ± 0.6 95–99 189 ± 75 20–518
   CD14 (n = 6) 1.6 ± 0.6 0.2–4.9 83 ± 32 22–255
   CD68 (mAb PG-M1) (n = 3) 0.5 ± 0.0 0.5–0.6 29 ± 6 19–40
(e)OA-SFB (negative isolation from primary culture; negative fraction)
   Thy-1 (n = 9) 62.1 ± 9.0 28–92 120 ± 36 53–404
   Prolyl-4-hydroxylase (n = 7) 92.2 ± 2.8 77–98 212 ± 44 30–382
   CD14 (n = 9) 1.3 ± 0.3 0.7–3.0 268 ± 97 14–754
   CD68 (mAb PG-M1) (n = 7) 1.3 ± 0.2 0.6–2.1 58 ± 18 14–140
(f)OA-SFB (fourth-passage; conventionally passaged cells)
   Thy-1 (n = 3) 80.9 ± 5.0 72–93 56 ± 6 46–70
   Prolyl-4-hydroxylase (n = 3) 98.8 ± 0.3 98–99 250 ± 27 205–313
   CD14 (n = 3) 1.0 ± 0.5 0.2–2.2 24 ± 1 22–26
(g) Primary culture or first-passage normal skin-FB
   Thy-1 (n = 8) 91.4 ± 3.0 78–99 156 ± 36 34–349
   Prolyl-4-hydroxylase (n = 8) 70.7 ± 15.2 2–99 101 ± 31 10–269
   CD14 (n = 8) 1.5 ± 0.4 0.3–4.0 70 ± 14 22–130
   CD68 (mAb PG-M1) (n = 5) 0.5 ± 0.3 0.1–1.4 82 ± 25 12–164
  1. mAb, Monoclonal antibody.