Table 1 Composition of rheumatoid arthritis (RA)-synovial fibroblasts (SFB), osteoarthritis (OA)-SFB, and normal skin-FB, as determined by flow cytometry for various numbers of patients/donors
Percent of positive cells | Mean fluorescence intensity | |||
|---|---|---|---|---|
Composition | Mean ± SEM | Range | Mean ± SEM | Range |
(a) Primary-culture RA synovial cells | ||||
Thy-1 (CD90) (n = 4) | 62.0 ± 14.6 | 24–93 | 152 ± 49 | 46–283 |
CD14 (n = 4) | 14.8 ± 6.0 | 2–25 | 230 ± 87 | 64–445 |
(b)RA-SFB (negative isolation from primary culture; negative fraction) | ||||
Thy-1 (n = 9) | 73.8 ± 9.6 | 21–99 | 109 ± 20 | 53–222 |
Prolyl-4-hydroxylase (n = 9) | 84.8 ± 2.9 | 66–95 | 164 ± 45 | 20–391 |
CD14 (n = 9) | 0.7 ± 0.2 | 0.1–1.7 | 62 ± 12 | 30–126 |
CD68 (mAb PG-M1) (n = 5) | 1.7 ± 0.6 | 0.4–3.2 | 120 ± 78 | 10–430 |
(c)RA-SFB (fourth-passage; isolated cells) | ||||
Thy-1 (n = 3) | 86.6 ± 4.6 | 79–95 | 41 ± 7 | 27–53 |
Prolyl-4-hydroxylase (n = 3) | 98.7 ± 0.8 | 97–100 | 164 ± 62 | 82–285 |
CD14 (n = 3) | 0.6 ± 0.2 | 0.2–1.0 | 50 ± 30 | 20–110 |
(d)RA-SFB (fourth-passage; conventionally passaged cells) | ||||
Thy-1 (n = 6) | 85.9 ± 3.9 | 70–95 | 95 ± 15 | 43–167 |
Prolyl-4-hydroxylase (n = 6) | 98.3 ± 0.6 | 95–99 | 189 ± 75 | 20–518 |
CD14 (n = 6) | 1.6 ± 0.6 | 0.2–4.9 | 83 ± 32 | 22–255 |
CD68 (mAb PG-M1) (n = 3) | 0.5 ± 0.0 | 0.5–0.6 | 29 ± 6 | 19–40 |
(e)OA-SFB (negative isolation from primary culture; negative fraction) | ||||
Thy-1 (n = 9) | 62.1 ± 9.0 | 28–92 | 120 ± 36 | 53–404 |
Prolyl-4-hydroxylase (n = 7) | 92.2 ± 2.8 | 77–98 | 212 ± 44 | 30–382 |
CD14 (n = 9) | 1.3 ± 0.3 | 0.7–3.0 | 268 ± 97 | 14–754 |
CD68 (mAb PG-M1) (n = 7) | 1.3 ± 0.2 | 0.6–2.1 | 58 ± 18 | 14–140 |
(f)OA-SFB (fourth-passage; conventionally passaged cells) | ||||
Thy-1 (n = 3) | 80.9 ± 5.0 | 72–93 | 56 ± 6 | 46–70 |
Prolyl-4-hydroxylase (n = 3) | 98.8 ± 0.3 | 98–99 | 250 ± 27 | 205–313 |
CD14 (n = 3) | 1.0 ± 0.5 | 0.2–2.2 | 24 ± 1 | 22–26 |
(g) Primary culture or first-passage normal skin-FB | ||||
Thy-1 (n = 8) | 91.4 ± 3.0 | 78–99 | 156 ± 36 | 34–349 |
Prolyl-4-hydroxylase (n = 8) | 70.7 ± 15.2 | 2–99 | 101 ± 31 | 10–269 |
CD14 (n = 8) | 1.5 ± 0.4 | 0.3–4.0 | 70 ± 14 | 22–130 |
CD68 (mAb PG-M1) (n = 5) | 0.5 ± 0.3 | 0.1–1.4 | 82 ± 25 | 12–164 |