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Table 4 Phenotype of negatively isolated rheumatoid arthritis (RA)-synovial fibroblasts (SFB) in comparison with osteoarthritis (OA)-SFB and normal skin-FB

From: Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture — primary culture cells markedly differ from fourth-passage cells

  skin-FB (n = 8) OA-SFB (n = 9) RA-SFB (n = 9)
Antigen Percent MFI Percent MFI Percent MFI
Thy-1 (CD90; mAb AS02) 91.4 ± 3.0 156 ± 36 62.1 ± 9.0# 120 ± 36 73.8 ± 9.6 109 ± 20
MHC-I 90.2 ± 8.5 414 ± 119 c87.7 ± 6.8 759 ± 170 a77.2 ± 8.1 130 ± 54x
MHC-II 1.5 ± 0.4 225 ± 67 c17.0 ± 7.3# 678 ± 137# a65.8 ± 12.3#x 223 ± 84x
Aminopeptidase N (CD13) b84.0 ± 10.2 365 ± 163 a83.2 ± 8.7 794 ± 207 84.8 ± 5.9 444 ± 226
VCAM-1 (CD 106) c8.3 ± 3.4 66 ± 33 a28.9 ± 13.2 156 ± 82 c17.5 ± 9.8 122 ± 42
Prolyl-4-hydroxylase 70.7 ± 15.2 101 ± 31 a92.2 ± 2.8 212 ± 44 84.8 ± 2.9 164 ± 45
Vimentin a90.3 ± 4.2 133 ± 34 b94.2 ± 1.7 1083 ± 530 a93.2 ± 1.5 592 ± 290
Procollagen I d68.2 ± 8.3 18 ± 1 d27.5 ± 10.4 39 ± 5# a44.8 ± 13.0 25 ± 2x
Procollagen III d55.1 ± 9.8 15 ± 2 d22.1 ± 7.8 45 ± 9# a50.0 ± 14.3 26 ± 3#
c-Fos d87.2 ± 2.1 17 ± 3 d53.9 ± 10.4 35 ± 2# a57.4 ± 14.0 20 ± 2x
c-Jun d42.7 ± 15.6 13 ± 3 d22.2 ± 10.3 29 ± 3 b42.5 ± 14.0 21 ± 5
Jun-D d87.4 ± 1.5 19 ± 5 d37.6 ± 17.7 33 ± 1# a53.0 ± 14.8 21 ± 4
  1. Data presented as means ± SEM. MFI, Mean fluorescence intensity; mAb, monoclonal antibody; MHC, major histocompatibility complex; VCAM-1, vascular adhesion molecule-1. Significant differences among groups are indicated in bold. All cell populations were isolated/obtained from primary culture (in the case of skin-FB from primary culture or first passage; see Methods for details). #P ≤ 0.05 Mann-Whitney U test versus skin-FB. xP ≤ 0.05 Mann-Whitney U test versus OA-SFB. an = 7 Normals, OA or RA patients. bn = 6 Normals, OA or RA patients. cn = 5 Normals, OA or RA patients. dn = 3 Normals or OA patients.