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Table 4 Phenotype of negatively isolated rheumatoid arthritis (RA)-synovial fibroblasts (SFB) in comparison with osteoarthritis (OA)-SFB and normal skin-FB

From: Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture — primary culture cells markedly differ from fourth-passage cells

 

skin-FB (n = 8)

OA-SFB (n = 9)

RA-SFB (n = 9)

Antigen

Percent

MFI

Percent

MFI

Percent

MFI

Thy-1 (CD90; mAb AS02)

91.4 ± 3.0

156 ± 36

62.1 ± 9.0#

120 ± 36

73.8 ± 9.6

109 ± 20

MHC-I

90.2 ± 8.5

414 ± 119

c87.7 ± 6.8

759 ± 170

a77.2 ± 8.1

130 ± 54x

MHC-II

1.5 ± 0.4

225 ± 67

c17.0 ± 7.3#

678 ± 137#

a65.8 ± 12.3#x

223 ± 84x

Aminopeptidase N (CD13)

b84.0 ± 10.2

365 ± 163

a83.2 ± 8.7

794 ± 207

84.8 ± 5.9

444 ± 226

VCAM-1 (CD 106)

c8.3 ± 3.4

66 ± 33

a28.9 ± 13.2

156 ± 82

c17.5 ± 9.8

122 ± 42

Prolyl-4-hydroxylase

70.7 ± 15.2

101 ± 31

a92.2 ± 2.8

212 ± 44

84.8 ± 2.9

164 ± 45

Vimentin

a90.3 ± 4.2

133 ± 34

b94.2 ± 1.7

1083 ± 530

a93.2 ± 1.5

592 ± 290

Procollagen I

d68.2 ± 8.3

18 ± 1

d27.5 ± 10.4

39 ± 5#

a44.8 ± 13.0

25 ± 2x

Procollagen III

d55.1 ± 9.8

15 ± 2

d22.1 ± 7.8

45 ± 9#

a50.0 ± 14.3

26 ± 3#

c-Fos

d87.2 ± 2.1

17 ± 3

d53.9 ± 10.4

35 ± 2#

a57.4 ± 14.0

20 ± 2x

c-Jun

d42.7 ± 15.6

13 ± 3

d22.2 ± 10.3

29 ± 3

b42.5 ± 14.0

21 ± 5

Jun-D

d87.4 ± 1.5

19 ± 5

d37.6 ± 17.7

33 ± 1#

a53.0 ± 14.8

21 ± 4

  1. Data presented as means ± SEM. MFI, Mean fluorescence intensity; mAb, monoclonal antibody; MHC, major histocompatibility complex; VCAM-1, vascular adhesion molecule-1. Significant differences among groups are indicated in bold. All cell populations were isolated/obtained from primary culture (in the case of skin-FB from primary culture or first passage; see Methods for details). #P ≤ 0.05 Mann-Whitney U test versus skin-FB. xP ≤ 0.05 Mann-Whitney U test versus OA-SFB. an = 7 Normals, OA or RA patients. bn = 6 Normals, OA or RA patients. cn = 5 Normals, OA or RA patients. dn = 3 Normals or OA patients.