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Figure 2 | Arthritis Res Ther

Figure 2

From: Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+T cells from patients with rheumatoid arthritis

Figure 2

(a) Interferon gamma (IFN-γ) production by CD3 and CD28 costimulated CD4+ T cells in the presence of IL-10 in patients with rheumatoid arthritis (RA) and in healthy controls (HC). CD4+ T cells (5 × 105 cells in 0.5 ml culture medium with 10% FCS) were stimulated by anti-CD3 antibody and anti-CD28 antibody with or without 1 ng/ml IL-10. Concentrations of IFN-γ in culture supernatants were measured in duplicate by ELISA. RA patients were divided into those with active disease (multiple inflammatory joints and CRP level ≥ 10 mg/l) and inactive disease (in remission and CRP level ≤ 4 mg/l). The results are represented as a box plot; upper and lower bars, 90th and 10th percentiles, respectively; upper, center and lower lines of box, 75th, 50th, and 25th percentiles, respectively. (b) Percentage of IFN-γ production. IFN-γ production with IL-10 expressed as % IFN-γ production without IL-10. Values are the mean ± standard error of the mean. n, number of samples tested.

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