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Figure 6 | Arthritis Res Ther

Figure 6

From: Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+T cells from patients with rheumatoid arthritis

Figure 6

(a) The mRNA expression of SOCS1 and SOCS3 in CD4+ T cells from patients with RA and healthy controls (HC). Total cellular RNA was extracted from freshly isolated CD4+ T cells and mRNA expression of SOCS1 and SOCS3 was analyzed by real time-PCR as described in Patients and Methods. Levels of SOCS1 and SOCS3 mRNAs were normalized relative to β-actin expression. Values are the mean ± SEM. n = number of samples tested. (b) Kinetics of IL-6-induced SOCS1 mRNA expression in normal CD4+ T cells. CD4+ T cells from HC (5 × 105 cells in 0.5 ml of culture medium with 10% FCS) were stimulated with IL-6 (10 ng/ml) and SOCS1 mRNA expression was determined at the indicated time after stimulation.

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Arthritis Research & Therapy

ISSN: 1478-6362

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