p53tumor suppressor gene mutations in fibroblast-like synoviocytes from erosion synovium and non-erosion synovium in rheumatoid arthritis

Table 2 p53 mutations in paired rheumatoid arthritis (RA) fibroblast-like synoviocytes and synovial tissue samples

Patient	Fibroblast-like synoviocytes		Synovial tissue
	Mutation	Frequency	Mutation	Frequency
RA10	Codon 119 GCC>ACC (Ala>Thr)	1/10	Codon 196 CGA>TGA (Arg>stop)	1/12
	Codon 178 CAC>AC (deletion)	1/10
	Codon 223 CCT>CAT (Pro>His)	1/10
	Codon 360 GGG>AGG (Gly>Arg)	1/10
	Long deletion codon 143 – codon 220	1/10
	Codon 213 CGA>CGG (Arg>Arg)	5/10^a	Codon 213 CGA>CGG (Arg>Arg)	6/12
RA11	Codon 307 GCA>GCT (Ala>Ala)	2/9	Codon 147 GTT>ATT (Val>IIe)	1/12
	Deletion codon 304 – codon 337	1/9	Codon 225 GTT>GTG (Val>Val)	1/12
RA12	Codon 355 GCT>GCC (Ala>Ala)	1/12	Codon 213 CGA>CAA (Arg>Gln)	1/11
			Codon 213 CGA>CGG (Arg>Arg)	1/11
			Codon 269 AGC>AAC (Ser>Asn)	1/11
RA13	Codon 134 TTT>CTT (Phe>Leu)	1/12	None	0/11
	Codon 216 GTG>ATG (Val>Met)	1/12
	Codon 249 AGG>GGG (Arg>Gly)	3/12

Data presented as number of mutant subclones/total number of subclones analyzed. Nested PCR required for RA12 and RA13 FLS and RA10 – RA13 synovial tissues. ^a Recognized p53 polymorphism also detected in normal peripheral blood mononuclear cells (most probably germline).

ISSN: 1478-6362