The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin

Table 1 The range of IgG concentrations (ng/ml) produced by expression of the 32 heavy chain/light chain combinations

Heavy chain	Light chain contributing CDR1	Light chain contributing CDR2 and CDR3	Light chain name	IgG concentration (ng/ml)
IS4	IS4	IS4	IS4	24–368
IS4	IS4	B3	IB	22–140
IS4	IS4	UK4	IU	70–194
IS4	B3	B3	B3	5–14
IS4	B3	IS4	BI	50–60
IS4	B3	UK4	BU	5–60
IS4	UK4	UK4	UK4	11–22
IS4	UK4	IS4	UI	50–480
IS4	UK4	B3	UB	9–50
B3	IS4	IS4	IS4	71–192
B3	IS4	B3	IB	41–96
B3	IS4	UK4	IU	89–376
B3	B3	B3	B3	3.5–6
B3	B3	IS4	BI	120–608
B3	B3	UK4	BU	40–68
B3	UK4	UK4	UK4	8–28
B3	UK4	IS4	UI	60–480
B3	UK4	B3	UB	2–20
IS4	B3(Arg27aSer)	B3	B3a	48–60
B3	B3(Arg27aSer)	B3	B3a	2.5–4
IS4V_Hi	IS4	IS4	IS4	50–56
IS4V_Hii	IS4	IS4	IS4	65–70
IS4V_Hiii	IS4	IS4	IS4	48–90
IS4V_Hiv	IS4	IS4	IS4	48–90
IS4V_Hx	IS4	IS4	IS4	78–94
IS4V_Hi&ii	IS4	IS4	IS4	74–80
IS4V_Hi	B3	B3	B3	24–54
IS4V_Hii	B3	B3	B3	30
IS4V_Hiii	B3	B3	B3	30–34
IS4V_Hiv	B3	B3	B3	28–30
IS4V_Hx	B3	B3	B3	32–34
IS4V_Hi&ii	B3	B3	B3	32–47

IgG concentrations in COS-7 cell supernatants were determined by ELISA. The hybrid light chains were named by combining the names of the two parent antibodies such that the first letter represented the antibody from which the complementarity determining region (CDR) 1 was derived and the last letter represented the antibody from which both the CDR2 and CDR3 were derived. At least two expression experiments were carried out for each combination; identical concentrations were obtained for IS4V_Hii/B3V_L from two different expression experiments.

ISSN: 1478-6362