Table 1 TaqMan® probes and primers for the genes of interest
Gene of interest | Forward primer | Reverse primer | Probe |
|---|---|---|---|
IL-1β | 5'-CACCTCTCAAGCAGAGCACAG | 5'-GGGTTCCATGGTGAAGTCAAC | 5'-6-FAM-TGTCCCGACCATTGCTGTTTCCTAGG-TAMRA |
IL-6 | 5'-CAAGACCATCCAACTCATCTTG | 5'-CACAGTGAGGAATGTCCACAAAC | 5'-6-FAM-TCGGCAAACCTAGTGTGCTATGCCTAAGCA-TAMRA |
TNF-α | 5'-CCAGGTTCTCTTCAAGGGACAA | 5'-CTCCTGGTATGAAATGGCAAATC | 5'-6-FAM-CCCGACTATGTGCTCCTCACCCACA-TAMRA |
GRO1 | 5'-TGTGTTGAAGCTTCCCTTGGA | 5'-TGAGACGAGAAGGAGCATTGGT | 5'-6-FAM-TGTCTAGTTTGTAGGGCACAATGCCCT-TAMRA |
CD14 | 5'-GGACGAGGAAAGTGTCCGCT | 5'-AGGTACTCCAGGCTGCGACC | 5'-6-FAM-TTCTATGCGCGGGGGCGGAA-TAMRA |
CD3 | 5'-GGATGGAGTTCGCCAGTCAA | 5'-GGTTTCCTTGGAGACGGCTG | 5'-6-FAM-ACAGGTCTACCAGCCCCTCAAGGACCG-TAMRA |
Ubiquitin | 5'-CGAGAACGTGAAGGCCAAGA | 5'-GGAGGACAAGGTGCAGGGTT | 5'-6-FAM-CCCCTGACCAGCAGAGGCTCATCTTTG-TAMRA |