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Figure 2 | Arthritis Res Ther

Figure 2

From: Local expression of matrix metalloproteinases, cathepsins, and their inhibitors during the development of murine antigen-induced arthritis

Figure 2

Real-time polymerase chain reaction (PCR) analyses of matrix metalloproteinase (MMP), cathepsin and pro-inflammatory cytokine expression in murine arthritic knee joints. Total RNA was isolated from murine right knee joints before (d0) and after (d1–d21) arthritis induction. mRNA samples from three (n = 3) (days 1, 7 and 21) or five (n = 5) (days 0, 3 and 14) animals were analyzed by real-time PCR with Rotorgene 2000. The mean of day 0 expression was set at 100%. The housekeeping gene β-actin was used for normalization of expression. Significant changes in comparison with day 0 expression are indicated by asterisks (increased expression) [(*) P ≤ 0.1, * P ≤ 0.05, ** P ≤ 0.01] or hash signs (decreased expression) [(#) P ≤ 0.1, # P ≤ 0.05]. Results are expressed as means and SEM. (a) MMP expression; (b) cathepsin expression; (c) pro-inflammatory cytokine expression. IFN, interferon; IL, interleukin; TNF, tumor necrosis factor.

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