Skip to main content
Figure 5 | Arthritis Res Ther

Figure 5

From: Local expression of matrix metalloproteinases, cathepsins, and their inhibitors during the development of murine antigen-induced arthritis

Figure 5

Detection, by gelatin zymography, of pro-matrix metalloproteinase-2 (pro-MMP-2) and pro-MMP-9 expression in joint extracts at different time points after the induction of antigen-induced arthritis. Joint extracts were prepared at days 0, 1, 3, 7 and 21 and from normal mice (N) (three individual animals per day). The protein concentration was determined by bicinchoninic acid protein assay, and equal amounts of protein were applied to each lane. The zymography was developed and stained as described in Materials and Methods. The picture shows a representative gel of three comparable experiments. S, standard (human pro-MMP-2, [72 kDa] and pro-MMP-9 [92 kDa]).

Back to article page