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Table 1 Real-time polymerase chain reaction primers for analysis of proteinase, proteinase inhibitor and cytokine mRNA expression

From: Local expression of matrix metalloproteinases, cathepsins, and their inhibitors during the development of murine antigen-induced arthritis

Gene

Forward primer

Reverse primer

Product size (base pairs)

IFN-γ

5'-gcg tca ttg aat cac acc tg-3'

5'-gac ctg tgg gtt gtt gac ct-3'

104

IL-1β

5'-cag gca ggc agt atc act ca-3'

5'-atg agt cac aga gga tgg gc-3'

140

IL-4

5'-agc tgc aga gac tct ttc gg-3'

5'-tgc tct tta ggc ttt cca gg-3'

111

IL-6

5'-ccg gag agg aga ctt cac ag-3'

5'-cag aat tgc cat tgc aca ac-3'

134

IL-17

5'-cct aag aaa ccc cca cgt tt-3'

5'-ttc ttt tca ttg tgg agg gc-3'

129

TNF-α

5'-acg gca tgg atc tca aag ac-3'

5'-gtg ggt gag gag cac gta gt-3'

116

MMP-2

5'-agc gtg aag ttt gga agc at-3'

5'-cac atc ctt cac ctg gtg tg-3'

105

MMP-3

5'-tgg aga tgc tca ctt tga cg-3'

5'-gcc ttg gct gag tgg tag ag-3'

120

MMP-9

5'-cat tcg cgt gga taa gga gt-3'

5'-att ttg gaa act cac acg cc-3'

118

MMP-12

5'-ttt gga gct cac gga gac tt-3'

5'-cac gtt tct gcc tca tca aa-3'

116

MMP-13

5'-agt tga cag gct ccg aga aa-3'

5'-ggc act cca cat ctt ggt tt-3'

105

TIMP-1

5'-ggt gtt tcc ctg ttt atc-3'

5'-tag ttc ttt att tca cca tct-3'

254

TIMP-3

5'-ttg ggt acc ctg gct atc ag-3'

5'-agg tct ggg ttc agg gat ct-3'

132

Cathepsin B

5'-gga gat act ccc agg tgc aa-3'

5'-ctg cca tga tct cct tca ca-3'

121

Cathepsin H

5'-gg cag agc ctc aga att gc-3'

5'-act ggc gaa aca aca ttt gc-3'

109

Cathepsin K

5'-ggg cca gga tga aag ttg ta-3'

5'-cac tgc tct ctt cag ggc tt-3'

106

Cathepsin L

5'-atc ccc aag tct gtg gac tg-3'

5'-tca gtg aga tca gtt tgc cg-3'

145

Cathepsin S

5'-aga gaa ggg ctg cgt cac t-3'

5'-gat atc agc ttc ccc gtt ttc ag-3'

117

Cystatin B

5'-tgc tga caa ggt cag acc ac-3'

5'-gca acc acg tcc tac att ca-3'

133

β-actin

5'-cca cag ctg aga ggg aaa tc-3'

5'-tct cca ggg agg aag agg at-3'

108

  1. IFN, interferon; IL, interleukin; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinases; TNF, tumor necrosis factor.