Figure 3

CD11c⁺ dendritic cells (DCs) and CD123⁺ DCs are present in rheumatoid arthritis (RA) synovial fluid (SF). After purification of mononuclear cells from either normal or RA peripheral blood (PB) or RA SF, cells were stained with CD123-PE, CD11c FITC, CD14-PECy5, and HLA-DR-APC. Live HLA-DR⁺CD14^- mononuclear cells were gated; polymorphonuclear cells were excluded on the basis of forward and side light-scatter. Scatter plots depict percentage of total PB or SF cells expressing the indicated markers for all donors. APC, antigen-presenting cell; PBMC, peripheral blood mononuclear cells; PE, phycoerythrin; SFMC, synovial fluid mononuclear cells.