Figure 4
Dose-dependent effects of LY294002 or pyrrolidine dithiocarbamate (PDTC) in peripheral blood mononuclear cells (PBMC) from patients with rheumatoid arthritis (RA). (a) Effect of inhibitors on interleukin (IL)-17 release by anti-CD3-stimulated PBMC from patients with RA. (b) Effects of LY294002 or PDTC on PBMC viability. PBMC were cultured at a concentration of 2 × 105 cells per well with medium, anti-CD3, anti-CD3 and LY294002 or PDTC under the conditions described in the Materials and methods section. After 24 hours of treatment, cell viability was assessed by the trypan blue dye exclusion method and expressed as a percentage with the formula 100 × (number of viable cells/number of both viable and dead cells).