Table 1 Sequences of primers and probes for quantitative real-time polymerase chain reaction
From: Repression of anti-proliferative factor Tob1in osteoarthritic cartilage
Gene | GenBank accession no. | Primers (5'→3') | Conc. (nM) | Probe (5'→3') | MgCl2 (mM) |
|---|---|---|---|---|---|
GAPDH | NM_002046 | Forward: GAAGGTGAAGGTCGGAGTC | 50 | CAAGCTTCCCGTTCTCAGCC | 5.5 |
| Â | Â | Reverse: GAAGATGGTGATGGGATTTC | 900 | Â | Â |
TOB1 | NM_005749 | Forward: TCTGCTGCTGTAAGCCCTACCT | 300 | CGGTCCACTCAGCCTTTAACCTTTACCACT | 6.5 |
| Â | Â | Reverse: TTCATTTTGGTAGAGCCGAACTT | 900 | Â | Â |
Ki-67 | NM_002417 | Forward: CAGTGATCAACGCCGTAGGTC | 900 | CTTCCAGCAGCAAATCTCAGACAGAGGTTC | 6.0 |
| Â | Â | Reverse: TCGGCTGATAGACACTCTCTTTTG | 900 | Â | Â |
COL2A1 | NM_001844 | Forward:CAACACTGCCAACGTCCAGAT | 50 | ACCTTCCTACGCCTGCTGTCCACG | 5.5 |
| Â | Â | Reverse: CTGCTTCGTCCAGATAGGCAAT | 300 | Â | Â |
MMP-13 | NM_002427 | Forward: TCCTCTTCTTGAGCTGGACTCATT | 900 | TCCTCAGACAAATCATCTTCATCACCACCAC | 7.0 |
| Â | Â | Reverse: CGCTCTGCAAACTGGAGGTC | 50 | Â | Â |