Figure 4

Modulation of antigen-induced arthritis (AIA) by transfer of regulatory T cells (T_reg cells). Amelioration of clinical and histological severity of AIA by transfer of 2 × 10⁶ CD4⁺CD25⁺ cells freshly isolated from (a) naive or (b) immunized mice at the time of AIA induction (day 0; n = 6 per group). (c) Transfer of 1 × 10⁶ in vitro pre-activated cells at the time of AIA induction (n = 6). ^#P < 0.05, ^##P < 0.01 for CD4⁺CD25⁺ versus CD4⁺CD25^-; ⁺P < 0.05, ⁺⁺P < 0.01 for CD4⁺CD25⁺ versus phosphate-buffered saline. Dest, joint destruction; Inf, inflammatory changes; Score; total arthritis score