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Figure 1 | Arthritis Res Ther

Figure 1

From: Phenotypic and functional characterisation of CCR7+ and CCR7- CD4+memory T cells homing to the joints in juvenile idiopathic arthritis

Figure 1

Expression of CXCR3 and interferon (IFN)-γ by (SF) CCR7+ and CCR7- memory CD4+ cells from synovial fluid. IFN-γ expression was investigated by three-colour staining of freshly isolated SF CD45RO+ cells with CD4–fluorescein isothiocyanate (FITC), anti-CCR7–phycoerythrin (PE) and anti-CCR5–CyChrome monoclonal antibodies (mAbs) or CD4–TC (where TC stands for Tri-color), anti-CCR7–PE and anti-IFN-γ mAbs, respectively; CXCR3 expression was investigated by triple staining with CD4–TC, anti-CCR7–PE and anti-CXCR3–FITC, as described in the Methods section. Subsequently, cytofluorimetric analysis was performed by gating on the CD4+CCR7+ and CD4+CCR7- lymphocyte subsets. Data are expressed as percentages of positive cells or/and mean fluorescence intensity. (a, b) Expression of IFN-γ (a) and CXCR3 (b) by SF CCR7+ and CCR7- memory CD4+ cells from 10 patients with juvenile idiopathic arthritis (JIA). Boxes contain values falling between the 25th and 75th centiles; whiskers show lines that extend from the boxes represent the highest and lowest values for each subgroup. Differences between paired SF mononuclear cells were evaluated by the Wilcoxon rank test. (c, d) Dot plots show the cytofluorimetric analysis IFN-γ (c) and CXCR3 (d) expression by the gated CD4+CCR7+ (gate 1) and CD4+CCR7- (gate 2) cell populations in three representative patients with JIA.

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