Figure 2

Expression of CD4, CD20, CD45RO, CCR7, CCR5 and CXCR3 in synovial tissue obtained from patients with juvenile idiopathic arthritis (JIA) after synoviectomy. (a, b) Presence of CCR7⁺ cells (red) in the sublining layer of a synovial tissue characterised by a diffuse lymphocytic infiltrate (scattered CD4⁺ cells, brown) from a 14-year-old girl with antinuclear antibody-positive (ANA⁺) oligoarticular JIA (no. 1, Table 3) (Magnification × 20). (c–n) Serial stainings with CD20, CD4, CCR7, CXCR3 and CCR5 monoclonal antibody in synovial tissue from a 12-year-old girl with ANA⁺ oligoarticular JIA (no. 4, Table 3). The distribution of cells positive for CCR7, CCR5 and CXCR3 is shown in two different areas containing T and B cell aggregates (magnification × 10). (o, p) Different expression of CCR7 (red) and CCR5 (brown) in synovial membrane infiltrate (patient no. 1, Table 3) (magnification × 4.5). CCR7⁺ cells are observed exclusively in the perivascular lymphocytic infiltrate of the deep sublining layer (open rectangle). Conversely, CCR5-positive cells are prevalently observed at the level of the lining layer and superficial subintima (*) and in the perivascular infiltrates of the sublining layer (**).