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Figure 5 | Arthritis Research & Therapy

Figure 5

From: Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8

Figure 5

Blocking of monocyte migration induced by RA SCL supernatants with blocking anti-chemokine or chemokine receptor mAbs. Monocyte migration induced by supernatants of RA SCLs (RA6/1 and RA8/3) was assessed using mAbs, which can neutralize specific chemokines or chemokine receptors. Concentrations of mAbs were 10 μg/ml for MCP-1, IL-8, and IP-10, and 50 μg for anti-CCR5 mAb with 5 × 105 SRBC rosette negative cells. Migrating cells were stained with anti-CD-14 mAb, and migrating CD14+cells were counted by flow cytometry. The mean and SEM were calculated from the results of nine independent experiments. The paired Student t test was used to assess statistical differences versus the result with control mAb.

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