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Figure 6 | Arthritis Research & Therapy

Figure 6

From: Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8

Figure 6

Chemokine production by RA SCL stimulated with TNF-α. Production of MCP-1, IL-8, RANTES, IP-10, and MIP-1β was measured by ELISA. Various cell lines (1 × 105/ml) were cultured for 24 h in 2 ml DMEM containing 5% FBS with or without 2 ng/ml TNF-α, and supernatants were collected. The content of (A) MCP-1, (B) IL-8, (C) RANTES and (D) IP-10 are shown; MIP-1β was not detected (less than 10 pg/ml). RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from triplicates of one representative experiment out of three with similar results. Statistical analysis was performed with the Student t test. *P < 0.05 and **P < 0.01.

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