Figure 8
Blocking of monocyte migration induced by supernatant of RA SCL stimulated with TNF-α. Monocyte migration induced by supernatants of RA SCLs (RA6/1 and RA8/3) stimulated with 2 ng/ml TNF-α was assessed using mAbs, which can neutralize specific chemokines or chemokine receptors. Concentrations of mAbs were 10 μg/ml for MCP-1, IL-8, and IP-10, and 50 μg for anti-CCR5 mAb with 5 × 105 SRBC rosette negative cells. Migrating cells were stained with anti-CD-14 mAb, and migrating CD14+cells were counted by flow cytometry. The mean and SEM were calculated from the results of three independent experiments. The paired Student t test was used to assess statistical differences versus the result with control mAb.