Figure 4

C3 has no role in mediating inflammatory responses in zymosan-induced arthritis. (a) ^99mTc uptake measurement showed an attenuation of inflammation at early and late time points in TLR2/C3 double-deficient (KO) mice (n = 5) compared with double wild-type (WT) mice (n = 5) but did not reach statistical significance. (b) Histological scoring showed a significant decrease in both cell infiltration (P < 0.05) and cartilage destruction (P < 0.05) in TLR2/C-3 double-deficient mice (n = 5) compared with littermate control (n = 5). (c) Production of specific anti-zymosan IgGs was reduced in double-deficient mice in comparision with double WT littermates. Values correspond to the ratio of zymosan-specific IgG to total IgG mentioned in Fig. 2c.