Phenotypic characteristics of human monocytes undergoing transendothelial migration

Table 3 Changes in surface phenotypes of migrated monocytes

Marker	TNF-α	IFN-γ	IL-1α	MIP-1α
CD11a	96 ± 8	106 ± 16	76 ± 41	87 ± 9
CD33	74 ± 49	105 ± 7	107 ± 16	165 ± 107
CD45RA	52 ± 53	134 ± 77	73 ± 65	63 ± 34
CD45RB	91 ± 7	86 ± 4	79 ± 44	84 ± 23
CD45RO	80 ± 9^*	98 ± 6	92 ± 8	78 ± 54
CD49d	92 ± 31	83 ± 26	81 ± 18	87 ± 21
CD54	199 ± 55^*	111 ± 38	173 ± 27^*	76 ± 31
CD62L	88 ± 10	52 ± 39	105 ± 42	103 ± 16
CD86	103 ± 25	219 ± 134	69 ± 25	90 ± 36
HLA-DR	86 ± 2^*	88 ± 18	87 ± 20	82 ± 52

The changes in individual surface markers after pretreatment of endothelial cells (ECs) with the indicated cytokines and chemokines are shown as percentages of control experiments with untreated ECs. All results were derived from at least three independent experiments for each cytokine or chemokine. HLA-DR, human leucocyte antigen-DR; IFN-γ, interferon-γ; IL-1α, interleukin-1α; MIP-1α, macrophage inflammatory protein-1α; TNF-α; tumour necrosis factor-α. ^*Statistically significant change (P < 0.05) compared with cells that migrated through untreated ECs.

ISSN: 1478-6362