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Table 1 Proportion of regulatory T cells to the total CD4+ T cell population in lymphoid organs of naive and immunised IFN-γ receptor knock-out and wild-type (WT) DBA/1 mice

From: Defective CD4+CD25+regulatory T cell functioning in collagen-induced arthritis: an important factor in pathogenesis, counter-regulated by endogenous IFN-γ

    100 × CD4+CD25+/CD4+ (N)
Treatment Expt no. Organ IFN-γR KO WT
Naive 1 Thymus 3.2 ± 0.6 (5) 2.2 ± 0.7 (5)
   Spleen 10.1 ± 0.9 (3) * 7.4 ± 0.2 (3)
   Lymph nodes 6.9 ± 1.1 (5) 5.1 ± 1.1 (5)
  2 Spleen 14.4 (1) 9.9 (1)
   Lymph nodes 9.1 ± 0.9 (4) 6.6 ± 0.7 (4)
  3 Lymph nodes 11.2 (1) 7.0 (1)
CIA 4 Thymus 3.5 ± 0.9 (3) 4.0 ± 1.6 (3)
   Spleen 11.0 ± 1.3 (2) 7.9 ± 0.7 (2)
   Lymph nodes 10.2 ± 0.8 (6) 7.7 ± 0.6 (6)
  5 Spleen 12.1 ± 2.9 (3) 9.2 ± 0.8 (3)
   Lymph nodes 12.9 ± 1.4 (4) 10.3 ± 1.1 (4)
  6 Lymph nodes 13.4 ± 0.4 (4) * 9.2 ± 0.7 (4)
  1. Cells were obtained from thymuses, spleens or lymph nodes of IFN-γ receptor knock-out (IFN-γR KO) and wild-type DBA/1 mice. In experiments 4 to 6, mice were immunised with collagen type II in complete Freund's adjuvant on day 0, and cells were obtained on day 21 (collagen-induced arthritis; CIA). Cells were stained with anti-CD25-FITC and phycoerythrin-conjugated anti-CD4 antibodies. The proportion of CD4+CD25+ in the total CD4+ T cell population is shown. In experiments 1, 2, 4 and 5, N (number in parentheses) indicates the number of mice in each experiment; in experiments 3 and 6, N represents the number of experiments, each consisting of groups of 5 to 10 mice, from which samples were pooled for analysis. *Significant difference between IFN-γR KO and wild-type mice (P < 0.05; Mann–Whitney U-test).