Skip to main content

Table 3 Effect of IL-13 on MMP mRNA levels in synovium of mice with ICA

From: Local IL-13 gene transfer prior to immune-complex arthritis inhibits chondrocyte death and matrix-metalloproteinase-mediated cartilage matrix degradation despite enhanced joint inflammation

 

ICA day 3

ICA day 7

 

AxCANI

AxCAhIL-13

AxCANI

AxCAhIL-13

MMP-3

5.7 ± 0.3

7.1 ± 0.8

4.2 ± 0.5

6.1 ± 0.2*

MMP-9

5.1 ± 0.2

4.8 ± 0.3

0.2 ± 0.6

3.9 ± 0.5*

MMP-12

0.6 ± 0.4

5.8 ± 1.1*

0.9 ± 1

8.1 ± 0.9*

MMP-13

3.2 ± 0.2

2.7 ± 0.3

4.3 ± 0.3

6.4 ± 0.3*

MMP-14

3.7 ± 0.4

4.9 ± 0.8

3.7 ± 1

3.7 ± 0.6

  1. Expression profile of MMP-3, -9, -12, -13, and -14 mRNA levels after injection of AxCANI or AxCAhIL-13 in synovial biopsies isolated at day 3 and day 7 after arthritis onset. The Ct values for MMP genes in naive knee joints were subtracted from the Ct values for MMPs at day 3 and 7 after arthritis onset. Ct values were corrected for GAPDH content for each individual sample. Note that MMP-3, -9, -12, and -13 mRNA levels were significantly increased at day 7 by IL-13, and the MMP-12 mRNA level was already elevated at day 3. Values represent means ± SEM for 5 mice. *P < 0.05, Mann–Whitney U test. AxCAhIL-13 = adenovirus encoding interleukin-13; AxCANI = adenovirus encoding no gene; Ct = cycle threshold; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; ICA = immune-complex-mediated arthritis; MMP = matrix metalloproteinase.