Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis

Table 3 Changes (fold change relative to day 0) in cytokine protein concentrations during rat AIA

Time point	IL-1β	IL-6	TNF-α	IL-2	IFN-γ	IL-4	IL-10
Inguinal lymph node
Day 0	1.00 (0.27)	1.00 (0.26)	1.00 (0.26)	1.00 (0.31)	1.00 (0.28)	1.00 (0.25)	1.00 (0.20)
6 hours	1.67 (0.60)	0.98 (0.38)	1.93 (0.78)	1.06 (0.31)	0.98 (0.25)	1.38 (0.55)	1.60 (0.32)
Day 1	1.74 (0.58)	0.59 (0.42)	2.53 (0.78)	1.31 (0.45)	1.14 (0.34)	1.86 (0.72)	2.18* (0.49)
Day 3	1.24 (0.29)	0.40 (0.21)	1.38 (0.28)	0.66 (0.12)	0.55 (0.11)	0.53 (0.28)	1.41 (0.24)
Day 6	1.86* (0.41	0.10* (0.10)	1.87 (0.38)	0.69 (0.14)	0.81 (0.12)	0.91 (0.27)	0.89 (0.17)
Spleen
Day 0	1.00 (0.12)	1.00 (0.41)	1.00 (0.10)	1.00 (0.23)	1.00 (0.13)	1.00 (0.12)	1.00 (0.05)
6 hours	0.75 (0.09)	0.32 (0.14)	0.61* (0.04)	0.70 (0.07)	0.78 (0.04)	0.77 (0.04)	0.94 (0.06)
Day 1	0.72 (0.11)	0.41 (0.17)	0.95 (0.13)	0.84 (0.16)	0.81 (0.07)	0.80 (0.08)	0.87 (0.08)
Day 3	0.81 (0.06)	0.24 (0.14)	1.07 (0.08)	0.70 (0.13)	0.71 (0.06)	0.85 (0.06)	0.90 (0.06)
Day 6	0.67 (0.06)	0.82*⁺ (0.07)	1.36 (0.21)	1.10*⁺ (0.13)	0.91⁺ (0.06)	0.96 (0.10)	0.99 (0.07)

Values (fold changes) are means (standard error of the mean) (n = 5 to 6 rats for each time point). AIA, antigen-induced arthritis. *P ≤ 0.05 in comparison with day 0; ⁺P ≤ 0.05 in comparison with the preceding date.

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