Figure 4

P2X₇-stimulated lymphocyte programmed cell death (PCD). Lymphocytes from New Zealand Black (NZB) mice, New Zealand White (NZW) mice and (NZB × NZW)F₁ (NZB/W) mice were stained with anti-CD4^APC and anti-CD8^FITC, and were equilibrated with propidium iodide (PI). Panels show the mean percentage (± standard deviation, n = 5) of dead cells (those taking up PI) as assessed by flow cytometry before (t = 0), and at 15-min intervals subsequent to, stimulation of the P2X₇ receptor with 2'-3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate. (a) CD4⁺ cells, and (b) CD8⁺ cells. NZB, open squares; NZW, open circles; NZB/W, open diamonds.