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Table 3 Effect of synovial microparticles on the release of inflammatory mediators by fibroblast-like synoviocytes from arthritic patients (n = 11)

From: Synovial microparticles from arthritic patients modulate chemokine and cytokine release by synoviocytes

Mediator Control P* MP-free synovial fluid MP (1×) Nx/Nt P MP (3×) Nx/Nt P
  Unstimulated IL-1β         
MCP-1 (pg/ml) 456 4,754 0.001 469 488 6/11 0.010 900 4/6 0.156
  (355–1,292) (2,492–6,081)   (293–1,241) (338–1,481) higher   (346–2,326) higher  
sICAM-1 (ng/ml) 0.09 0.40 0.007 1.04 2.00 8/11 0.010 6.07 6/6 0.031
  (0–0.3) (0–0.76)   (0.34–1.84) (0.35–4.07) higher   (0.91–11.75) higher  
IL-8 (pg/ml) 0 8,642 0.001 26 301 5/11 0.008 790 6/6 0.031
  (0–564) (2,954–18,330)   (0–528) (0–707) higher   (0–2,100) higher  
IL-6 (pg/ml) 74 4949 0.001 110 136 7/11 0.042 436 6/6 0.031
  (24–1,710) (1,870–22,797)   (30–1,176) (34–1,937) higher   (44–3,766) higher  
VEGF (pg/ml) 48 79 0.014 34 74 10/11 0.001 111 6/6 0.078
  (11–102) (7–141)   (1–97) (28–138) higher   (27–161) higher  
GM-CSF (pg/ml) 32 53 0.004 31 22 10/11 0.002 18 6/6 0.016
  (28–40) (40–72)   (26–70) (14–43) lower   (14–25) lower  
RANTES (pg/ml) 0 138 0.001 0 0.2 4/11 0.031 4.2 5/6 0.062
  (0–74) (46–277)   (0–58) (0–86) higher   (0–32) higher  
  1. Results are medians, with ranges in parentheses. Concentrations of mediators were determined in the culture supernatant of the fibroblast-like synoviocytes (FLS) by ELISA as described in the Materials and methods section. FLS were incubated for 24 hours with 1 ml of culture medium containing 1% FCS (negative control), 975 μl of culture medium supplemented with either (1) 25 μl of interleukin (IL)-1β (final concentration 125 pg/ml; positive control), (2) 25 μl (onefold (1×) or threefold (3×) concentrated) microparticles (MP), or (3) MP-free synovial fluid. P*, positive versus negative control; P, MP (1×) versus MP-free synovial fluid; P, MP (3×) versus MP (1×). Nx/Nt, number of individual culture supernatants that contained elevated or decreased concentrations of mediators after incubation for 24 hours with isolated MP compared with MP-free synovial fluid, divided by the number of patients studied. GM-CSF, granulocyte/macrophage colony-stimulating factor; sICAM-1, soluble intracellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; VEGF, vascular endothelial growth factor.