Effect of leptin/IL-1 co-stimulation on nitric oxide synthase (NOS) type II mRNA expression. Real-time RT-PCR analysis of the expression of the inducible NOS type II mRNA in leptin (OB)/IL-1 co-stimulated ATDC5 cells. Stimulations (24 hours) were conducted in serum-free conditions. Specific inhibitors were added 1 hour before cytokine co-stimulation. Values are expressed as mean ± standard error of the mean.