Figure 4

Biochemical analysis of pellets. Results are shown of the measurement of cartilage matrix protein accumulation in day 14 pellets normalized to cell number (DNA) in cells from the unaffected (filled bars) and affected (open bars) sides of four consecutive donors; the sex and age (in years) of the donors are indicated. Glycosaminoglycan (GAG) (a) and hydroxyproline (as a measure of total collagen content) (b) are shown as amounts per microgram of DNA. Results are means ± SD for three identical pellets. The amounts are compared with the mean value for four sequential autologous chondrocyte transplantation patients (ACT). An asterisk indicates a significant difference (P < 0.05) between ACT and osteoarthritis chondrocytes.