Figure 5

Immunoprecipitation of SAA in SJIA, resulting in loss of the 11.4- to 11.6-kDA peak cluster. A pooled sample from four sera before conventional treatment (top panel) was incubated with either Protein A–Sepharose beads alone (middle panel) or Protein A–Sepharose beads bound with anti-SAA antibody (lower panel). After immunoprecipitation, the depleted serum was subjected to SELDI-TOF MS. The 11.4-to 11.6-kDa m/z peak cluster is shown in grey. MRA, humanized anti-IL-6 receptor monoclonal antibody; SAA, serum amyloid A; SELDI-TOF MS, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry; SJIA, systemic-onset juvenile idiopathic arthritis.