Skip to main content

Table 1 Results of the technical comparison of indirect immunofluorescence (IIF), immunoblot and ELISA for the detection of anti-polymyositis/scleroderma (anti-PM/Scl) antibodies

From: Clinical evaluation of autoantibodies to a novel PM/Scl peptide antigen

Sample number IIF Immunoblot ELISA
  Titer Pattern PM/Scl-75 PM/Scl-100 Other PM/Scl-100 PM1-α
1 1:10000 FG, N ++ +++   15.3 17.7
2 1:1000 FG, N + +   2.9 7.6
3 1:3200 FG, N ++ ++   14.4 29.2
  1:320 AMA      
4 1:320 FG, N - +   1.1 4.7
5 1:1000 FG, N + +   2.2 12.1
6 1:3200 FG, N - -   0.6 2.3
7 1:1000 FG, N ++ +++   5.2 13.3
8 1:1000 FG, N + +   2.3 12.7
9 1:3200 FG, N + +++ P38 15.5 36.1
10 -   - +   1.7 4.0
11 1:1000 FG, N - ++   19.2 2.4
12 1:1000 FG, N ++ +   1.2 3.2
13 1:3200 FG, N +++ +++ Ku86, Jo-1 6.5 10.6
14 1:3200 FG, N - ++   2.1 5.9
15 1:3200 FG, N ++ +++   11.4 22.5
16 1:1000 FG, N - +   3.6 8.5
17 1:1000 FG, N + ++   3.2 15.0
18 1:3200 FG, N ++ ++   6.1 11.8
19 n.d. n.d. - ++   7.1 19.4
20 1:320 N - +   2.1 5.6
21 n.d. n.d. +++ ++   4.1 12.8
22 1:10000 FG - + P38 0.7 1.5
  1:10000 SPA      
23 1:1000 N n.d. n.d.   0.5 2.7
24 n.d. n.d. ++ +++   18.2 34.7
25 1:10000 N n.d. n.d. P38 0.5 2.0
  1:1000 Rib      
26 1:1000 N n.d. n.d.   1.9 8.4
27 n.d. n.d. + ++   5.9 19.4
28 n.d. n.d. ++ +++   20.9 37.3
29 1:1000 Hom n.d. n.d. Ku86, Cen, M2 0.6 0.8
  1:100 N*      
30 1:1000 N - +   0.6 1.4
31 1:3200 Hom, N - -   0.5 2.9
32 1:3200 N - -   0.5 1.3
33 1:320 FG, N + ++   7.2 25.7
Number positive/tested   26/29 17/29 27/29   26/33 32/33
  1. -, negative; +, weak positive; ++, positive; +++, strong positive; n.d., not determined; FG, fine granular; Hom, homogenous; SPA, spindle apparatus; N, nucleoli; AMA, anti-mitochondrial antibodies; Cen, centromere; Rib, ribosomal. * Primate liver.