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Table 1 PCR primers and sequence start position for amplification of MT-ND1 and DLD

From: Somatic mutations in the mitochondria of rheumatoid arthritis synoviocytes

Primer name

Start position

Sequence 5'-3'

mtMT-ND1a

  

   F1A

2995

TTGGATCAGGACATCCCGA

   R1A

3645

ACGGCTAGGCTAGAGGTGG

   F1B

3536

TTAGCTCTCACCATCGCT

   R1B

4239

ATTGTAATGGGTATGGAGACA

   F2

4184

TTCCTACCACTCACCCTAG

   R2

4869

CATGTGAGAAGAAGCAG

DLD b

  

   DLD – sense

417

ATGATGGAGCAGAAGAGTACTGCA

   DLD – antisense

1088

TTTAGTTTGAAATCTGGTATTGAC

  1. aSee Fig. 1 for position of primers. Both forward and reverse primers in addition to the specific nucleotide sequence have a corresponding M13 tag (M13F, 5'-TGTAAAACGACGGCCAGT- 3' ; M13R, 5'-CAGGAAACAGCTATGACC-3'); start position numbering represents location of 5' end corresponding to the Anderson Reference Sequence [17]. bStart position numbering represents location of the 5' end and corresponds to the DLD cDNA numbering system published by Pons and colleagues [19]. mt, mitochondrial.