Table 1 Primers used for RT-PCR
Gene | Annealing temperature (°C) | Product size (base pairs) | Sequence (5' to 3') | GenBank accession number |
|---|---|---|---|---|
Collagen II | 65 | 141 | F ACGGTGGACGAGGTCTGACT R GGCCTGTCTCTCCACGTTCA | AF138883 |
Aggrecan | 65 | 375 | F CCGCTATGACGCCATCTGCT R TGCACGACGAGGTCCTCACT | AF019758 |
Decorin | 55 | 319 | F CAAACTCTTTTGCTTGGGCT R CACTGGACAACTCGCAGATG | AF125041 |
Biglycan | 65 | 204 | F CCATGCTGAACGATGAGGAA R CATTATTCTGCAGGTCCAGC | AF034842 |
Fibromodulin | 65 | 442 | F CTGGACCACAACAACCTGAC R GGATCTTCTGCAGCTGGTTG | AF020291 |
Lumican | 65 | 284 | F CAGCCATGTACTGCGATGAG R CTGCAGGTCCACCAGAGATT | NM173934 |
TGF-β | 60 | 271 | F CGGCAGCTGTACATTGACTT R AGCGCACGATCATGTTGGAC | AF000133 |
CTGF | 65 | 504 | F TCTTCTGCGACTTCGGCTCC R CCTCCAGGTCAGCTTCGCAA | NM174030 |
Collagen I | 65 | 460 | F CCACCAGTCACCTGCGTACA R GGAGACCACGAGGACCAGAA | AF129287 |
Collagen III | 55 | 243 | F GCTGGCTAGTTGTCGCTCTG R GTGGGGAAACTGCACAACAT | L47641 |
GAPDH | 55 | 320 | F TCACCATCTTCCAGGAGCGA R GGCGTGGACAGTGGTCATAA | AF035421 |