Skip to main content

Table 1 Numbers of positive cells before and after intervention

From: Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis

Cell type Treatment Observer 1 t0 Observer 1 t1 Intra-observer comparison Observer 2 Observer 3 Inter- observer comparison
   Placebo Prednisolone P a Placebo Prednisolone P a P b Placebo Prednisolone P a Placebo Prednisolone P a Pc
CD3+ T lymphocytes Before 192 ± 246 358 ± 413 <0.05 110 ± 140 227 ± 225 <0.05 NS 179 ± 185 285 ± 250 <0.05 89 ± 110 112 ± 98 <0.05 NS
  After 387 ± 391 140 ± 150   299 ± 356 44 ± 62    470 ± 618 101 ± 94   196 ± 224 54 ± 66   
CD38+ plasma cells Before 56 ± 87 99 ± 130 NS 73 ± 134 116 ± 166 NS NS 246 ± 307 397 ± 498 NS 145 ± 151 309 ± 380 NS NS
  After 96 ± 127 37 ± 57   119 ± 149 42 ± 78    335 ± 411 132 ± 187   315 ± 416 83 ± 121   
CD68+ macrophages Before 804 ± 422 973 ± 419 <0.03 441 ± 422 572 ± 404 <0.03 NS 937 ± 292 1151 ± 254 <0.03 621 ± 445 724 ± 360 <0.03 NS
  After 972 ± 151 553 ± 342   632 ± 686 222 ± 278    984 ± 354 796 ± 306   720 ± 527 313 ± 291   
  1. Shown are the mean numbers (± standard deviation) of CD3+ T lymphocytes, CD38+ plasma cells and CD68+ sublining macrophages per square millimetre of synovial tissue before and after intervention, measured by one observer at two different time points (OB1 t0 and OB1 t1) and by two other observers (OB2 and OB3) for placebo-treated patients and prednisolone-treated patients. aNonparametric, unpaired, Mann–Whitney U-test for the comparison between placebo and prednisolone treatment. bNonparametric, paired, Wilcoxon signed rank test, for the comparison between OB1 t0 and OB1 t1 (intra-observer comparison). cNonparametric, paired, Friedman test, for the comparsion between the three observers (OB1 t0, OB2 and OB3).