mRNA levels of various MMPs and TIMPs in synovia derived from inflamed knee joints. Inflamed synovia were isolated 3 days (a,c) and 7 days (b,d) after IFN-γ-accelerated arthritis. The cycle threshold value (Ct) of the various MMP (a,b) and TIMP (c,d) genes was corrected for glyceraldehyde-3-phosphate dehydrogenase content and for values at zero time. Note that at day 7 significantly elevated levels of MMP-3, MMP-9 and MMP-13 were found in p47phox-/- mice in comparison with controls (b). TIMP-1 and 2 were also somewhat elevated, although to a lesser extent than MMP (d). No significant differences were found at day 3 (a,c).