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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Octacalcium phosphate crystals directly stimulate expression of inducible nitric oxide synthase through p38 and JNK mitogen-activated protein kinases in articular chondrocytes

Figure 4

OCP crystal-induced iNOS mRNA expression and NO release is not IL-1β dependent. Nonadherent articular chondrocytes were stimulated with OCP crystals or IL-1β for 24 hours with or without pretreatment with IL-1ra for 1 hour. (a,b) IL-1β and iNOS transcripts were assessed by RT-PCR and (c) NO release was measured in the supernatants. The RT-PCR was representative of three experiments. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1ra, IL-1 receptor antagonist; iNOS, inducible nitric oxide synthase; NO, nitric oxide; OCP, octacalcium phosphate.

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Arthritis Research & Therapy

ISSN: 1478-6362

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