Figure 7
Distribution of proteoglycans in the explants cultured with CII-C1 or F(ab)2 from CII-C1. Toluidine blue stained sections cultured for 14 days with (a) CII-C1 or (d) F(ab)2 are shown alongside (b, e) chemical maps showing proteoglycan distribution and (c, f) FTIRM spectra from the central areas (red line) and near the surface of the tissue (blue line). Note that the proteoglycan levels are lower and the amide 1 peak has shifted across the whole of the F(ab)2 treated tissue. The error bars represent 1 standard deviation at those points in the spectra.