Figure 2

Amino acid sequences of expressed Vλ regions compared with their closest germline λ gene, 2a2. The amino acid sequences of B3V_L, B3(R27aS)V_L, B33V_L, BUV_L, 33.H11V_L, and UK-4V_L regions are numbered according to the system of Wu and Kabat [26]. Amino acids are indicated by their one-letter code. Dots have been inserted to facilitate the alignment. A dash indicates sequence identity with that of germline gene 2a2. The complementarity-determining regions (CDRs) and framework regions (FRs) have been defined according to the system of Wu and Kabat [26]. Antigen contact sites, as defined by MacCallum and colleagues [27], are shown by red arrows. L1, L2 and L3 are the first, second and third contact regions of the light chain respectively.