Accelerated development of arthritis in mice lacking endothelial selectins

Table 1 Cytokine levels

Cytokine	Genotype	Cytokine level	P (versus E/P-selectin mutant)	Number of observations
IL-12	Wild-type
	Nonimmunized	2.2 ± 0.2 pg/mg	>0.05	12
	Pre-onset	ND	ND	0
	Post-onset	1.6 ± 0.2 pg/mg	>0.05	8
	Chronic	2.6 ± 0.6 pg/mg	>0.05	8
	E/P-selectin mutant
	Nonimmunized	5.7 ± 3.1 pg/mg		10
	Pre-onset	ND		0
	Post-onset	1.7 ± 0.2 pg/mg		8
	Chronic	2.3 ± 0.5 pg/mg		8
TNF-α^a	Wild-type
	Nonimmunized	<1 pg/mg	NS	10
	Pre-onset	<1 pg/mg		13
	Post-onset	<1 pg/mg		8
	Chronic	<1 pg/mg		8
	E/P-selectin mutant
	Nonimmunized	<1 pg/mg		10
	Pre-onset	<1 pg/mg		11
	Post-onset	<1 pg/mg		7
	Chronic	<1 pg/mg		8
MIP-2	Wild-type
	Nonimmunized	2.2 ± 0.4 pg/mg	>0.05	12
	Pre-onset	1.9 ± 0.9 pg/mg	>0.05	12
	Post-onset	5.3 ± 0.9 pg/mg	>0.05	8
	Chronic	1.4 ± 0.1 pg/mg	>0.05	10
	E/P-selectin mutant
	Nonimmunized	1.6 ± 0.2 pg/mg		10
	Pre-onset	2.4 ± 1.8 pg/mg		9
	Post-onset	5.2 ± 1.6 pg/mg		8
	Chronic	1.7 ± 0.5 pg/mg		9

Cytokines were not elevated in murine joint homogenates from nonimmunized E/P-selectin mutant mice, or from pre-CIA onset, post-CIA onset, and chronic-CIA (day 80) E/P-selectin mutant mice, compared with wild-type mice. Values for TNF-α are below the sensitivity of the assay. ELISA assay sensitivities were 2.5 pg/ml for IL-12, 5 pg/ml for TNF-α and IL-1β, and 1.5 pg/ml for MIP-2 and MIP-1α. Pre-onset group corresponds to combined values for day 16, day 22, and day 28 groups. All values are normalized to milligrams of total protein. CIA, collagen-immunized arthritis; MIP, macrophage inflammatory protein; ND, no data.

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