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Table 1 Cytokine levels

From: Accelerated development of arthritis in mice lacking endothelial selectins

Cytokine Genotype Cytokine level P (versus E/P-selectin mutant) Number of observations
IL-12 Wild-type    
     Nonimmunized 2.2 ± 0.2 pg/mg >0.05 12
     Pre-onset ND ND 0
     Post-onset 1.6 ± 0.2 pg/mg >0.05 8
     Chronic 2.6 ± 0.6 pg/mg >0.05 8
  E/P-selectin mutant    
     Nonimmunized 5.7 ± 3.1 pg/mg   10
     Pre-onset ND   0
     Post-onset 1.7 ± 0.2 pg/mg   8
     Chronic 2.3 ± 0.5 pg/mg   8
TNF-αa Wild-type    
     Nonimmunized <1 pg/mg NS 10
     Pre-onset <1 pg/mg   13
     Post-onset <1 pg/mg   8
     Chronic <1 pg/mg   8
  E/P-selectin mutant    
     Nonimmunized <1 pg/mg   10
     Pre-onset <1 pg/mg   11
     Post-onset <1 pg/mg   7
     Chronic <1 pg/mg   8
MIP-2 Wild-type    
     Nonimmunized 2.2 ± 0.4 pg/mg >0.05 12
     Pre-onset 1.9 ± 0.9 pg/mg >0.05 12
     Post-onset 5.3 ± 0.9 pg/mg >0.05 8
     Chronic 1.4 ± 0.1 pg/mg >0.05 10
  E/P-selectin mutant    
     Nonimmunized 1.6 ± 0.2 pg/mg   10
     Pre-onset 2.4 ± 1.8 pg/mg   9
     Post-onset 5.2 ± 1.6 pg/mg   8
     Chronic 1.7 ± 0.5 pg/mg   9
  1. Cytokines were not elevated in murine joint homogenates from nonimmunized E/P-selectin mutant mice, or from pre-CIA onset, post-CIA onset, and chronic-CIA (day 80) E/P-selectin mutant mice, compared with wild-type mice. Values for TNF-α are below the sensitivity of the assay. ELISA assay sensitivities were 2.5 pg/ml for IL-12, 5 pg/ml for TNF-α and IL-1β, and 1.5 pg/ml for MIP-2 and MIP-1α. Pre-onset group corresponds to combined values for day 16, day 22, and day 28 groups. All values are normalized to milligrams of total protein. CIA, collagen-immunized arthritis; MIP, macrophage inflammatory protein; ND, no data.