Skip to main content
Figure 5 | Arthritis Research & Therapy

Figure 5

From: Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production

Figure 5

Matrix metalloproteinase (MMP) secretion in a co-culture of THP-1 cells and human fibroblast cells (HFC). (a) Gel zymography detection of MMP-2 and MMP-9 in undifferentiated THP-1 cells, in HFC, and in a co-culture of the two. Elevated release and activation of MMP-2 and especially MMP-9 was seen in the co-culture cells compared to the cultures of THP-1 cells or HFC alone. (b) Gel zymography detection of MMP-2 and MMP-9 in phorbol myristate acetate (PMA)-induced differentiated THP-1 cells, in HFC, and in a co-culture of the two. Pro-MMP-2 and MMP-2 release was higher in the co-cultured cells. PMA-induced differentiated THP-1 cells secreted more pro-MMP-9 and MMP-9 compared with undifferentiated THP-1 cells. (c) Effects of CD147 antagonistic peptide 9 (AP9) on MMP-9 release in a co-culture of undifferentiated THP-1 cells and HFC. The zymography gels above were scanned and the gray density of strips was counted. The release and activation of MMP-9 was significantly reduced when AP9 was added. The asterisk indicates a p-value < 0.01. (d) Effects of AP9 on the release of pro-MMP-9, MMP-9 and MMP-2 in a co-culture of differentiated THP-1 cells and HFC. Pro-MMP-9, MMP-9 and MMP-2 secretion all decreased significantly in the AP9 group. Asterisks indicate a p-value < 0.01. HFC, Human fibroblast cells.

Back to article page