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Table 1 Primer design for quantitative RT-PCR analysis

From: The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanases

mRNA Primersa
MMP-13 Fw: 5'-TTGGTCAGATGTGACACCTC
Rv: 5'-ATCGGGAAGCATAAAGTGGC
Cathepsin K Fw: 5'-AGGTGGATGAAATCTCTCGG
Rv: 5'-TTCTTGAGTTGGCCCTCCAG
5-LOX Fw: 5'-TGCGTTCCAGTGACTTCCAC
Rv: 5'-CTCTGCACCATCTGCACGTG
ADAMTS-4 Fw: 5'-TACTACTATGTGCTGGAGCC
Rv: 5'-AGTGACCACATTGTTGTATCC
ADAMTS-5 Fw: 5'-GGCATCATTCATGTGACAC
Rv: 5'-GCATCGTAGGTCTGTCCTG
GAPDH Fw: 5'-AGGCTGTGGGCAAGGTCATC
Rv: 5'-AAGGTGGAAGAGTGGGTGTC
  1. aFw, forward; Rv, reverse. GAPDH, glyseraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase.