The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanases

Table 1 Primer design for quantitative RT-PCR analysis

mRNA	Primers^a
MMP-13	Fw: 5'-TTGGTCAGATGTGACACCTC Rv: 5'-ATCGGGAAGCATAAAGTGGC
Cathepsin K	Fw: 5'-AGGTGGATGAAATCTCTCGG Rv: 5'-TTCTTGAGTTGGCCCTCCAG
5-LOX	Fw: 5'-TGCGTTCCAGTGACTTCCAC Rv: 5'-CTCTGCACCATCTGCACGTG
ADAMTS-4	Fw: 5'-TACTACTATGTGCTGGAGCC Rv: 5'-AGTGACCACATTGTTGTATCC
ADAMTS-5	Fw: 5'-GGCATCATTCATGTGACAC Rv: 5'-GCATCGTAGGTCTGTCCTG
GAPDH	Fw: 5'-AGGCTGTGGGCAAGGTCATC Rv: 5'-AAGGTGGAAGAGTGGGTGTC

^aFw, forward; Rv, reverse. GAPDH, glyseraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase.

ISSN: 1478-6362