Figure 3

Apoptosis evaluation in cultured human THP-1 cell line. (a) Apoptosis evaluation in the cultured human THP-1 cell line after 168 hours of hormonal treatment without and with staurosporine. Results are expressed as the mean ± standard deviation of the percentage number of annexin-positive cells in five different experiments. (b) Apoptotic DNA evaluation in THP-1 cells after 168 hours of hormonal treatment without staurosporine as detected by flow analysis cytometry scatter analysis. Region G, control (cnt), 3.96%; 17β-oestradiol (E2), 5.54%; testosterone (T), 15.9%. (c) Apoptotic DNA evaluation in THP-1 cells after 168 hours of hormonal treatment and staurosporine (stau) for 24 hours, as detected by flow analysis cytometry scatter analysis. Region G: control/S, 18.9%; E₂/S, 16.5%; T/S, 24.5%. PI, propidium iodide.