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Figure 6 | Arthritis Research & Therapy

Figure 6

From: Sex hormone modulation of cell growth and apoptosis of the human monocytic/macrophage cell line

Figure 6

Electrophoretic mobility shift assay for NF-κB (arrow) in the cultured human THP-1 cell line. (a) Electrophoretic mobility shift assay for NF-κB (arrow) in the cultured human THP-1 cell line after 168 hours of hormonal treatment and without or with staurosporine (17 nM) reveals an increased DNA binding in 17β-oestradiol (E2)-treated cells. (b) Super shift assay for p65 (arrow) in the cells under the same conditions confirms the increased binding of p65 in E2-treated cells. The results are representative of four separate experiments. cnt, control; T, testosterone.

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