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Table 1 NF-κB complex, IκB-α and IκB-α-ser 32 evaluated by immunocytochemistry

From: Sex hormone modulation of cell growth and apoptosis of the human monocytic/macrophage cell line

 

NF-κB

IκB-α

IκB-α-ser 32

Without staurosporine

   

   Control

42.6 ± 4

17.7 ± 8

17.1 ± 2

   Testosterone (10 nM)

29.6 ± 5 (T vs control, P < 0.001)

26.9 ± 4 (T vs control, P < 0.01)

13 ± 3

   17β-oestradiol (10 nM)

45.8 ± 3 (E2 vs T, P < 0.001)

12.5 ± 2 (E2 vs T, P < 0.001)

25.3 ± 2 (E2 vs T, P < 0.01; E2 vs control, P < 0.001)

With staurosporine

   

   Control

18.9 ± 4

23 ± 6

11.6 ± 4

   Testosterone (10 nM)

18.2 ± 4

20.7 ± 3

9.2 ± 2

   17β-oestradiol (10 nM)

32.3 ± 6 (E2 vs T, P < 0.05; E2 vs control, P < 0.001)

25.5 ± 4 (E2 vs T, P < 0.05)

22.8 ± 3 (E2 vs T, P < 0.001; E2 vs control, P < 0.001)

  1. Expression and regulation after 168 hours of hormonal treatment (T, testosterone; E2, 17β-estradiol) in THP-1 cells (without and with staurosporine). Data are expressed as the mean ± standard deviation of the positive area per 100 cells stained for NF-κB complex, IκB-α and IκB-α-ser 32. Results were obtained as the mean of five different experiments. The statistical evaluation was determined for T versus control, for E2 versus control, and for E2 versus T (absence of significance not reported).
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Arthritis Research & Therapy

ISSN: 1478-6362

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