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Table 1 NF-κB complex, IκB-α and IκB-α-ser 32 evaluated by immunocytochemistry

From: Sex hormone modulation of cell growth and apoptosis of the human monocytic/macrophage cell line

  NF-κB IκB-α IκB-α-ser 32
Without staurosporine    
   Control 42.6 ± 4 17.7 ± 8 17.1 ± 2
   Testosterone (10 nM) 29.6 ± 5 (T vs control, P < 0.001) 26.9 ± 4 (T vs control, P < 0.01) 13 ± 3
   17β-oestradiol (10 nM) 45.8 ± 3 (E2 vs T, P < 0.001) 12.5 ± 2 (E2 vs T, P < 0.001) 25.3 ± 2 (E2 vs T, P < 0.01; E2 vs control, P < 0.001)
With staurosporine    
   Control 18.9 ± 4 23 ± 6 11.6 ± 4
   Testosterone (10 nM) 18.2 ± 4 20.7 ± 3 9.2 ± 2
   17β-oestradiol (10 nM) 32.3 ± 6 (E2 vs T, P < 0.05; E2 vs control, P < 0.001) 25.5 ± 4 (E2 vs T, P < 0.05) 22.8 ± 3 (E2 vs T, P < 0.001; E2 vs control, P < 0.001)
  1. Expression and regulation after 168 hours of hormonal treatment (T, testosterone; E2, 17β-estradiol) in THP-1 cells (without and with staurosporine). Data are expressed as the mean ± standard deviation of the positive area per 100 cells stained for NF-κB complex, IκB-α and IκB-α-ser 32. Results were obtained as the mean of five different experiments. The statistical evaluation was determined for T versus control, for E2 versus control, and for E2 versus T (absence of significance not reported).