Immunohistochemistry of ADAM15, vascular endothelial growth factor receptor (VEGFR)-2 and von Willebrand factor (vWF). (a-d) Rheumatoid arthritis (RA) synovial fibroblasts (SFs) and (e-h) human umbilical vein endothelial cells (HUVEC) were cultured on Lab-Tek II chamber slides and immunostained with antibodies against (a,e) ADAM15, (b,f) VEGFR-2 or (c,g) vWF or (d,h) non-immune mouse IgG as described in Materials and methods. Immunostaining of VEGFR-2 in RA SFs (b) was performed with RA SFs that were treated with 10 ng/ml TNF-α for 24 h prior to immunohistochemistry. Note that vWF is not immunostained in RA SFs (c), but VEGFR-2 is expressed in those stimulated with TNF-α (b). Scale bar, 25 μm.