Figure 6From: Expression of ADAM15 in rheumatoid synovium: up-regulation by vascular endothelial growth factor and possible implications for angiogenesisImmunoblotting of ADAM15 in rheumatoid arthritis (RA) synovial tissues. Homogenates of RA synovial tissues were prepared and subjected to immunoblotting with anti-ADAM15 antibody specific to the cysteine-rich domain of ADAM15 (240-2C7) (lane 2) or non-immune mouse IgG (lane 1) as described in Materials and methods. Immunoreactive bands of 100, 76, 66, 47, 41, 38, 34 and 29 kDa are indicated (arrow heads). The 58 kDa protein band is considered to be a non-specific band because it is also detected with non-immune IgG (lanes 1 and 2).Back to article page