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Table 1 Epitope sequences and epitope specificities of mAbs

From: Chronic development of collagen-induced arthritis is associated with arthritogenic antibodies against specific epitopes on type II collagen

Epitope

Epitope sequence (epitope in THP)a

MAbs

 

Antigen reactivityb

  

Clone

Isotype

CII

C1

J1

T1

U1

C1

GPBGPBGPBGPBGPBG-ARGLTGRBGDA-GPBGPBG-εACA

CIIC1

IgG2a

1.7

2.5

0.0

0.0

0.0

J1

GPBGPBGPBGPBGPBG-MBGERGAAGIAGPK-GPBGPBG-εACA

M2139

IgG2b

2.4

0.0

2.5

0.0

0.0

T1

GPBGPBGPBGPBGPBG-IAGFKGEQGPKGEP-GPBGPBG-εACA

122.9

IgG2a

1.7

0.0

0.0

2.5

0.0

U1

GPBGPBGPBGPBGPBG-LVGPRGERGFB-GPBGPBG-εACA

UL1

IgG2b

2.1

0.0

0.0

0.0

2.4

  1. aAll epitopes were in native triple-helical form. Amino acids are abbreviated as follows: G, glycine; P, proline; B, hydroxyproline; E, glutamic acid; R, arginine; K, lysine; H, histidine; F, phenylalanine; A, alanine; L, leucine; T, threonine; Y, tyrosine; εACA, ε-aminohexanoic acid-lysine-lysine-tyrosine-glycine-OH. bAbsorbance at 405 nm in ELISA tested with affinity-purified mAbs. In the ELISA plates, all the triple-helical peptides were coated at 4 μg/ml, whereas type II collagen (CII) was coated at 10 μg/ml. CIIC1, M2139 and UL1 are mouse monoclonal antibodies and were all tested at a concentration of 1 μg/ml; 122.9 is a rat monoclonal antibody and was tested with culture supernatant.